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Functional Genomic Analysis of Pseudorabies Virus: Functional Analysis of Pseudorabies Virus Genes by Real-time Rt Pcr and Knock-out Technique
Zsolt Boldogkoi
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Functional Genomic Analysis of Pseudorabies Virus: Functional Analysis of Pseudorabies Virus Genes by Real-time Rt Pcr and Knock-out Technique
Zsolt Boldogkoi
The pseudorabies virus (PRV) is a neurotropic herpesvirus, it is a pathogen of swine, causing Aujeszky's disease. PRV is a widely used model organism for studies in pathogenesis and molecular biology. This virus is also utilized as a neural circuit tracer. We have developed (1) a real-time RT-PCR method for the global analysis of PRV gene expression; (2) a novel calculation method to examine the relative expression ratios, and which is applicable for the analysis of gene expression in any genetic system that progressively changes in time; (3) PRV-based troponeon expressing transsynaptic tracers which label synaptically connected nerve cells; (4) transgenic viruses encoding differentially colored fluorescent proteins (FPs) and multiple colored FPs to differentiate the brain regions; (5) a method for a short-term culture of isolated cardiac muscle cells that retain their morphological and physiological integrity; (6) a PRV-based system for delivery of foreign genes to cardiomyocytes.
Medien | Bücher Taschenbuch (Buch mit Softcover und geklebtem Rücken) |
Erscheinungsdatum | 3. Januar 2011 |
ISBN13 | 9783843389488 |
Verlag | LAP LAMBERT Academic Publishing |
Seitenanzahl | 76 |
Maße | 226 × 5 × 150 mm · 122 g |
Sprache | Englisch |
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