Study of Bmp-2 and Stromal Cell Derived Factor-1 in Prostate Cancer

It was reported earlier that the exogenous BMP-2 treatment of two different primary fibroblastic cultures, each derived from a primary prostate tumor of the Pten null mouse model, results in enhanced secretion of SDF-1, a chemokine that functions as both chemoattractant for cell migration and mitogen for cell proliferation and survival. In the current study, we expanded the primary tumor or AD tumor CAF cultures by five more in number to determine how consistent the observed effect of BMP-2 is. While there is indeed a consistent enhancement of SDF-1 induction in all AD-CAF cultures, the degree of induction appears to vary widely. It is noteworthy that the single CRPC-CAF that we tested, however, appears to be distinct from the AD-CAFs in regard to two parameters. First, the CRPC-CAFs are found in repeated experiments, to naturally secrete a strong level of SDF-1, 4-fold higher than the best level seen in the AD-CAFs. Second, the induction of SDF-1 by BMP-2 is similarly increased in the CRPC CAFs. Thus it appears that the biological effect induced by BMP-2 is cell type dependent.